Investigative Approaches

Following is a list of approaches applied across the research areas:

Electrophysiology -In vitro and in vivo analysis of spiking activity, synaptic plasticity, circuit behavior, patch clamp, voltage clamp, single channel recording, current clamp recording of network activity via multi electrode arrays in vivo and in vitro, In vivo dialysis.

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Optogenetics and Live Imaging-learning, memory, behavior and sleep circuitry, confocal, multi-photon, total internal reflection microscopy, FRAP, FRET

Gene Expression and Proteomics-mass spec, LC-MS-MS, crystal structure, NMR, affinity purification, protein microarrays

Genetics and Genomics-mutagenic analysis via transgenic and knock-out organisms, real-time-PCR, RT-PCR, SNP typing, gene chip expression analysis and microarrays, CHiP analysis, transcriptome

Cell & Cell Culture Systems- primary cultures of neurons and glia, embryonic stem cells, induced pluripotent cells, stem brain slice, neuroendocrine cells 

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Model Systems-Caenorhabditis elegans, Drosophilia, Xenopus, Zebrafish, Chick, Mouse, Rat

Behavioral - operant conditioning, Morris water maze, Pavlovian conditioning, rotarod, elevated plus maze, social defeat, light-dark box, home cage activity, object recognition/discrimination, active/passive avoidance , operant conditioning for drug reward, conditioned place preference

Cognitive - structural and functional MRI, diffusion-weighted MRI, Transcranial magnetic stimulation, PET with various ligands, SPECT, genetic testing, neuropsychological assessments, behavioral testing